Case Studies

Below are a series of case studies highlighting how the MolecuLight i:X easily integrates into wound care protocols and guides clinicians in their wound care practice.


Featured Case Study

Real-time detection of asymptomatic bioburden with the MolecuLight i:X revealed need for systemic antibiotics and immediate hospitalization

Use of the MolecuLight i:X to image for bacterial fluorescence, in conjunction with clinical signs & symptoms, adds an additional bacterial-specific piece of information that can be captured and considered in real-time.

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Case Studies by Group


Allows clinicians to focus cleaning in areas where fluorescent bacteria are located and optimize wound bed preparation.

MolecuLight Clinical Case 0017

Improved cleaning with the MolecuLight i:X led to successful patient outcome

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Guides more efficient and targeted debridement.

MolecuLight Clinical Case 0045

Guided debridement with the MolecuLight i:X

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Guides where to sample; 50% more accurate swabbing compared to the Levine Technique.

There are no case studies currently.

Provides objective visual documentation of the presence of fluorescent bacteria and the surface area of the wound.

MolecuLight Clinical Case 0066

Document the path to wound healing with the MolecuLight i:X

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MolecuLight Clinical Case 0018

Greater measurement accuracy with the MolecuLight i:X led to improved tracking and documentation

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Treatment Selection

Comparing fluorescent bacteria and wound surface area at each visit may provide real-time objective feedback on impact of treatment plan.

MolecuLight Clinical Case 0048

Optimize Pseudomonas aeruginosa treatment with the MolecuLight i:X

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MolecuLight Clinical Case 0020b

Monitoring the effectiveness of an antibiotic against MRSA with the MolecuLight i:X

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Antibiotic Stewardship

Supports more responsible antibiotic decision making and selection.

MolecuLight Clinical Case 0047

Point of care identification of Pseudomonas aeruginosa with the MolecuLight i:X led to targeted treatment and wound healing

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Patient Engagement

Patients easily see and understand why a clinician is taking certain action to clean, debride and treat a wound in a specific way.

MolecuLight Clinical Case 0022

Improve patient adherence with the MolecuLight i:X

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  1. Wu YC, Smith M, Chu A, Lindvere-Teene L, Starr D, Tapang K, Wong O, Linden R, DaCosta RS. Handheld fluorescence imaging device detects subclinical wound infection in an asymptomatic patient with chronic diabetic foot ulcer: a case report. International Wound Journal, 2016, 13(4), 449-53. doi: 10.1111/iwj.12451.
  2. DaCosta RS, Kulbatski I, Lindvere-Teene L, Starr D, Blackmore K, Silver JI, Opoku J, Wu YC, Medeiros PJ, Xu W, Xu L, Wilson BC, Rosen C, Linden R. Point-of-care autofluorescence imaging for real-time sampling and treatment guidance of bioburden in chronic wounds: first-in-human results. PLOS ONE, 2015, 10(2). doi: 10.1371/journal.pone.0116623.
  3. Ottolino-Perry K, Chamma E, Blackmore KM, Lindvere-Teene L, Starr D, Tapang K, Rosen CF, Pitcher B, Panzarella T, Linden R, DaCosta RS. Improved detection of clinically relevant wound bacteria using autofluorescence image-guided sampling in diabetic foot ulcers. International Wound Journal, 2017, 14(5), 833-841. doi: 10.1111/iwj.12717.
  4. Rennie MY, Lindvere-Teene L, Tapang K, Linden R. Point-of-care fluorescence imaging predicts the presence of pathogenic bacteria in wounds: a clinical study. Journal of Wound Care, 2017, 26(8), 452-460. doi: 10.12968/jowc.2017.26.8.452.
  5. Raizman R. Point-of-care fluorescence imaging device guides care and patient education in obese patients with surgical site infections. Presented at CAWC 2016. Proceedings of the Annual Canadian Association of Wound Care Conference; 2016 Nov 3-6, Niagara Falls, ON, Canada.
  6. Jeffery S. Utility of point-of-care autofluorescence imaging device in successful closure of major limb amputations – a case study. Presented at MHSRS 2016. Proceedings of the Military Health System Research Symposium; 2016 Aug 15-18; Kissimmee, FL, USA.
  7. Dunham D, Teene L. Objective wound measurement software on a point-of-care, hand-held fluorescence imaging device: verification of measurement accuracy and repeatability. Presented at EWMA 2018. Proceedings of the Annual European Wound Management Association Conference; 2018 May 9-11; Krakow, Poland.
  8. Wu YC, Kulbatski I, Medeiros PJ, Maeda A, Bu J, Xu L, Chen Y, DaCosta RS. Autofluorescence imaging device for real-time detection and tracking of pathogenic bacteria in a mouse skin wound model: preclinical feasibility studies. Journal of Biomedical Optics, 2014, 19(8). doi: 10.1117/1.JBO.19.8.085002.
  9. Raizman R, DaCosta RS. Handheld real-time fluorescence imaging of bacteria guides treatment selection and timing of dressing changes in inpatients undergoing negative pressure wound therapy.  Presented at IWH 2016. Proceedings of the Innovations in Wound Healing Conference; 2016 Dec 8-11, Key Largo, FL, USA.
  10.  Landis S, Rennie MY, Blumenthal E, Jeffery S. Use of fluorescence imaging in visualizing bacteria in chronic ulcers and traumatic soft tissue damage. Presented at AMSUS 2016. Proceedings of the Annual Meeting of the Society of Federal Health Professionals; 2016 Nov 29-Dec 2; National Harbor, MD, USA.