Visualize Bacteria

See what you’re missing with MolecuLight i:

The MolecuLight i:X is a handheld fluorescence imaging device that provides instant visual detection and documentation of potentially harmful bacteria in wounds that would otherwise be invisible.1 The MolecuLight i:X emits a precise wavelength of safe violet light, which interacts with the wound tissue and bacteria causing the wound and surrounding skin to emit a green fluorescence (i.e. collagen) and potentially harmful bacteria to emit a red fluorescence (i.e. porphyrins).1 In real-time, MolecuLight i:X captures these red and green fluorescence signals using specialized optical components to filter out the violet light, and displays the resultant image immediately on the display screen (FL-image).1,2 The MolecuLight i:X is precisely calibrated to detect fluorescent bacteria at levels of ≥ 104 CFU/g on a quantitative scale or predominantly moderate to heavy growth on a semi-quantitative scale.3

Which types of bacteria can be detected?

Pre-clinical and clinical studies have shown that the MolecuLight i:X can detect red fluorescence from Gram positive, Gram negative, aerobic and anaerobic bacterial species1,5.

Pre-clinical research has demonstrated the following species can produce red fluorescence detectable by the MolecuLight i:X in vitro5 (see list below). However, there are many bacterial species not tested here that may also produce red fluorescence6.

  • Staphylococcus aureus
  • Staphylococcus epidermidis
  • Staphylococcus capitis
  • Staphylococcus lugdunensis
  • Pseudomonas aeruginosa
  • Pseudomonas putida
  • Escherichia coli
  • Corynebacterium striatum
  • Proteus mirabilis
  • Proteus vulgaris
  • Enterobacter cloacae
  • Serratia marcescens
  • Acinetobacter baumannii
  • Klebsiella pneumoniae
  • Klebsiella oxytoca
  • Morganella morganii
  • Propionibacterium acnes
  • Stenotrophonomas maltophilia
  • Bacteroides fragilis
  • Aeromonas hydrophilia
  • Alcaligenes faecalis
  • Bacillus cereus
  • Citrobacter koseri
  • Citrobacter freundii
  • Clostridium perfringens
  • Listeria monocytogenes
  • Peptostreptococcus anaerobius
  • Veillonella parvula


  1. Rennie, M. Y. et al. Understanding Real-Time Fluorescence Signals from Bacteria and Wound Tissues Observed with the MolecuLight i:X(TM). Diagnostics (Basel, Switzerland) 9, (2019).
  2. Rennie, M. Y., Lindvere-Teene, L., Tapang, K. & Linden, R. Point-of-care fluorescence imaging predicts the presence of pathogenic bacteria in wounds: a clinical study. J. Wound Care 26, 452–460 (2017).
  3. Hurley, C. M., McClusky, P., Sugrue, R. M., Clover, J. A. & Kelly, J. E. Efficacy of a bacterial fluorescence imaging device in an outpatient wound care clinic: a pilot study. J. Wound Care 28, 438–443 (2019).
  4. Serena, T. E., Harrell, K., Serena, L. & Yaakov, R. A. Real-time bacterial fluorescence imaging accurately identifies wounds with moderate-to-heavy bacterial burden. J. Wound Care 28, 346–357 (2019).
  5. Jones, L. M. et al. In Vivo and In Vitro Detection of Porphyrin-Producing Wound Pathogens, Planktonic and in Biofilm , with Real-Time Bacterial Fluorescence Imaging. Present. Wounds Canada (2019).